rabbit anti sirt3  (Cell Signaling Technology Inc)

Journal: Redox Biology

Article Title: SIRT3-mediated deacetylation of FoxM1 prevents pulmonary fibrosis via modulating the activation of pulmonary fibroblasts

doi: 10.1016/j.redox.2026.104108

Figure Lengend Snippet: Sirt3-dependent deacetylation of FoxM1 regulates the stability of FoxM1. (A) The Pearson's correlation analysis of COL1A1 expression with SIRTs expression based on the RNA-seq results of GSE2052 from GEO database. (B) Western blot analysis of SIRT3 expression in the lung tissues from bleomycin (BLM)-treated mice. n = 3, ∗ P < 0.05. (C) Western blot analysis of the acetylation levels of FoxM1 in SIRT3 flox/flox mice intratracheally injected with AAV-Cre. n = 3, ∗ P < 0.05. (D) Western blot analysis was performed to assess the acetylation status of FoxM1 in pulmonary fibroblasts following transfection with Sirt3 siRNA (si-Sirt3). n = 3, ∗ P < 0.05. (E) Western blot analysis of FoxM1 expression in CHX-treated pulmonary fibroblasts transfected with or without si-Sirt3. n = 3, ∗ P < 0.05. (F) Western blot analysis of CTHRC1, α-SMA and Collagen I expression in pulmonary fibroblasts transfected with or without si-Sirt3. n = 3, ∗ P < 0.05. (G) EdU assay for the proliferation of pulmonary fibroblasts transfected with or without si-Sirt3. n = 3, ∗ P < 0.05. (H) Western blot analysis of FoxM1 acetylation, CTHRC1, α-SMA, and Collagen I expression in TGF-β1-treated pulmonary fibroblasts transfected with or without LV-Sirt3. n = 3, ∗ P < 0.05. All data were presented as the means ± SEM. Paired t -test (B-D, F, G) and one-way ANOVA with Tukey's post-hoc test (E, H) were used for statistical analysis.

Article Snippet: The primary antibodies used were: FoxM1, α-SMA, SIRT3 and Lamin B1 (Selleckchem, Houston, USA), Collagen I (Proteintech, Wuhan, China) and GAPDH (ABclonal, Wuhan, China).

Techniques: Expressing, RNA Sequencing, Western Blot, Injection, Transfection, EdU Assay

Journal: Redox Biology

Article Title: SIRT3-mediated deacetylation of FoxM1 prevents pulmonary fibrosis via modulating the activation of pulmonary fibroblasts

doi: 10.1016/j.redox.2026.104108

Figure Lengend Snippet: Sirt3 knockdown accelerates BLM-induced pulmonary fibrosis via activation pulmonary fibroblasts in vivo. (A) Hematoxylin–eosin (H&E) and masson's trichrome staining for the lung tissues from SIRT3 flox/flox mice or BLM-treated SIRT3 flox/flox mice that intratracheally injected with or without AAV-Cre. (B, C) The ashcroft score (n = 6, ∗ P < 0.05.) and the hydroxyproline contents (n = 6, ∗ P < 0.05.) in the lung tissues of mice treated as in A. (D) Western blot analysis of CTHRC1, SIRT3, FoxM1, α-SMA and Collagen I expression in the lung tissues from mice treated as in A n = 3, ∗ P < 0.05. (E) Western blot analysis of CTHRC1, SIRT3, FoxM1, α-SMA and Collagen I expression in pulmonary fibroblasts isolated from mice treated as in A n = 3, ∗ P < 0.05. (F, G) EdU assay for the proliferation of pulmonary fibroblasts isolated from mice treated as in A. All data were presented as the means ± SEM. One-way ANOVA with Tukey's post-hoc test was used for statistical analysis.

Article Snippet: The primary antibodies used were: FoxM1, α-SMA, SIRT3 and Lamin B1 (Selleckchem, Houston, USA), Collagen I (Proteintech, Wuhan, China) and GAPDH (ABclonal, Wuhan, China).

Techniques: Knockdown, Activation Assay, In Vivo, Staining, Injection, Western Blot, Expressing, Isolation, EdU Assay

Journal: Redox Biology

Article Title: SIRT3-mediated deacetylation of FoxM1 prevents pulmonary fibrosis via modulating the activation of pulmonary fibroblasts

doi: 10.1016/j.redox.2026.104108

Figure Lengend Snippet: Nicotinamide riboside protects mice from bleomycin-induced pulmonary fibrosis via activation of SIRT3. (A) Western blot analysis of CTHRC1, SIRT3, FoxM1, α-SMA and Collagen I expression in TGF-β1-treated pulmonary fibroblasts accompany with or without NR treatment. n = 3, ∗ P < 0.05. (B) Cell viability assessment using the CCK-8 assay in pulmonary fibroblasts treated as in A n = 6, ∗ P < 0.05. (C) The survival of BLM-treated mice oral gavaged with or without NR. n = 18. (D) Hematoxylin–eosin (H&E) and masson's trichrome staining for the lung tissues from mice treated as in C. (E) The ashcroft score of mice treated as in C n = 6, ∗ P < 0.05. (F) Western blot analysis of CTHRC1, SIRT3, FoxM1, α-SMA and Collagen I expression in the lung tissues from mice treated as in C n = 3, ∗ P < 0.05. (G) Western blot analysis of CTHRC1, SIRT3, FoxM1, α-SMA and Collagen I expression in pulmonary fibroblasts isolated from the lung tissues of mice treated as in C n = 3, ∗ P < 0.05. All data were presented as the means ± SEM. One-way ANOVA with Tukey's post-hoc test was used for statistical analysis.

Article Snippet: The primary antibodies used were: FoxM1, α-SMA, SIRT3 and Lamin B1 (Selleckchem, Houston, USA), Collagen I (Proteintech, Wuhan, China) and GAPDH (ABclonal, Wuhan, China).

Techniques: Activation Assay, Western Blot, Expressing, CCK-8 Assay, Staining, Isolation

Journal: Journal of Inflammation Research

Article Title: The Interplay Among Gut Microbial Composition, Short-Chain Fatty Acid Metabolism and Gut-Kidney Oxidative Stress: Correlation with Diarrhea

doi: 10.2147/JIR.S588857

Figure Lengend Snippet: Changes in the relative expression levels of SIRT3 and SOD2 proteins in colon and kidney tissues of mice across different groups. ( A ) Western blot images of SIRT3 and SOD2 proteins in colon tissue; ( B ) Statistical analysis of the relative expression level of SIRT3 protein in colon tissue; ( C ) Statistical analysis of the relative expression level of SOD2 protein in colon tissue; ( D ) Western blot images of SIRT3 and SOD2 proteins in kidney tissue; ( E ) Statistical analysis of the relative expression level of SIRT3 protein in kidney tissue; ( F ) Statistical analysis of the relative expression level of SOD2 protein in kidney tissue. n = 3, ** P < 0.01. CC: normal group; CM: diarrhea model group.

Article Snippet: Adenine (purchased from Biofroxx, Germany, Cat. No.: 1163GR005), Folium sennae (obtained from Bozhou Huqiao Pharmaceutical Co., Ltd., Lot NO.: 2,311,300,022), Reactive Oxygen Species (ROS) Fluorescence Assay Kit (provided by Jiangsu Aidisheng Biotechnology Co., Ltd., Cat. No.: ADS-W-HY009), Superoxide Dismutase (SOD) Assay Kit (using the WST-8 method, from Jiangsu Aidisheng Biotechnology Co., Ltd., Cat. No.: ADS-W-KY011), Beta Actin Monoclonal Antibody (purchased from Wuhan Sanying Biotechnology, Inc., Cat. No.: 66,009-1-Ig), SIRT3 Polyclonal Antibody (from Wuhan Sanying Biotechnology, Inc, Cat. No.: 10,099-1-AP), SOD2 Polyclonal Antibody (from Wuhan Sanying Biotechnology, Inc., Cat. No.: 24,127-1-AP), HRP-labeled Goat Anti-Mouse Secondary Antibody (purchased from Wuhan Boster Biological Engineering Co., Ltd., Cat. No.: BA1051), and HRP-labeled Goat Anti-Rabbit Secondary Antibody (from Wuhan Boster Biological Engineering Co., Ltd., Cat. No.: BA1054).

Techniques: Expressing, Western Blot